SEMA3C Knockout cell line (HCT 116)

SEMA3C Knockout cell line (HCT 116) Order

Catalog#

YKO-XN44159

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SEMA3C Knockout cell line (HCT 116)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

SEMA3C

Gene ID

10512

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 2 (based on transcript SEMA3C-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SEMA3C gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SEMA3C gene knockout SEMA3C Knockout cell line (HCT 116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

SEMA3C Knockout cell line (HCT 116)

Catalog#

YKO-XN44159

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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SEMA3C Knockout cell line (HCT 116)

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